Transcriptome analysis reveals the important roles of a two-component system, flagellar assembly, active efflux system and outer membrane proteins in the anti-quinolone ability of Vibrio harveyi from orange-spotted grouper (<i>Epinephelus coioides</i>)

The drug resistance of Vibrio harveyi in aquaculture became more severe because the control Vibriosis depends majorly on current antibiotics. Transcriptomes a wild-type strain (VS) and its quinolone-resistant mutants (VR) V. were respectively sequenced by RNA-seq technology. A total 2,082 unigenes obtained after de novo splicing assembly. 129 genes identified with significant differential expression VR compared to VS, among which 65 up-regulated 64 down-regulated. Then, functional annotation enrichment analysis these differentially expressed (DEGs) performed. GO results showed that DEGs focused mainly cell structure, substance metabolism, transporter. COG classification amino acid transport wall/membrane biosynthesis, carbohydrate ribosomal biosynthesis. KEGG pathways related two-component system, ABC system flagellar assembly (ko02040) enriched significantly, 9 associated quinolone-resistance ability, including for resistance-related proteins, outer membrane DNA repair-related proteins discovered through drug-resistance genes. Ten (including above part genes) transcriptome data taken analyze their real-time qPCR. same as changes analysis, further confirming reliability sequencing analysis. In word, from assembly, active efflux take great roles . These provide enough information study molecular mechanism give helpful transcriptomic resource unravel contact between metabolic Vibrios